Rapid and Visual Detection of Monkey B Virus Based on Recombinase Polymerase Amplification

Objective: Monkey B virus (BV) infection in humans and other macaque species has a mortality rate of approximately 80%. Because BV infects through bites, scratches, injuries inflicted by macaques, the simple rapid diagnosis field laboratories is great importance to protect veterinarians, laboratory researchers, support personnels from threat infection. Methods: Two recombinase polymerase amplification (RPA) assays with closed vertical flow (VF) visualization strip (RPA-VF-UL27 RPA-VF-US6) were developed that target two conserved genes combined one-off, device. We compared sensitivities specificities after optimization reaction conditions. The performance RPA-VF-US6 at room temperature was determined evaluate its potential point-of-care (POC) testing. Result: specifically detected positive plasmid control (rather than nucleic acids herpesviruses) detection limit 28 copies, while RPA-VF-UL27 had cross-reactivity HSV-1, but even 3.4 copies standards readout this assay. Moreover, excellent (the 2,800 copies), indicating POC Conclusion: RPA for diagnosis. simple, rapid, specific method BV. entire can be performed constant within 30 min, suggesting testing without sophisticated instruments.